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| Costa,Eula Maria de M. B; Pimenta,Fabiana Cristina; Luz,Christian; Oliveira,Valéria de; Oliveira,Marília; Bueno,Elda; Petrofeza,Silvana. |
| Beauveria bassiana genetic diversity and ability to synthesize quercetin 2,3-dioxygenase (quercetinase) were analyzed. B. bassiana isolates, obtained from Brazilian soil samples, produced quercetinase after induction using 0.5 g/L quercetin. B. bassiana ATCC 7159 (29.6 nmol/mL/min) and isolate IP 11 (27.5 nmol/ml/min) showed the best performances and IP 3a (9.5 nmol/mL/min) presented the lowest level of quercetinase activity in the culture supernatant. A high level of polymorphism was detected by random amplified polymorphic DNA (RAPD) analysis. The use of internal-transcribed-spacer ribosomal region restriction fragment length polymorphism (ITS-RFLP) did not reveal characteristic markers to differentiate isolates. However, the ITS1-5.8S-ITS2 region... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Beauveria bassiana quercetinase quercetin-2; 3-dioxygenase genetic diversity. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100002 |
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| Luz,Christian; Silva,Ionizete G.; Magalhães,Bonifácio P.; Cordeiro,Celia M. T.; Tigano,Myrian S.. |
| Beauveria bassiana (Bals.) Vuill., isolate CG 306, was assayed against Triatoma infestans (Klug) using indirect application by contact with a treated filter paper. The fungus was deposited on filter paper by vacuum-filtration of a conidial suspension. Estimates of 50% survival time of insects were between 15 and 21 days at 3x10(6) and 10(7) conidia/cm² of treated support, respectively, without significant difference between concentrations. A concentration of 2.4x10(6) conidia/cm² of CG 306 was necessary to kill 50% of T. infestans third instar nymphs 25 days after inoculation at 25°C and 50% relative humidity, when insects were continuously exposed to conidia. The LC50 was significantly higher (2.0x10(7) conidia/cm²) when insects were exposed for 1 h to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Chagas disease; Biological control; Hyphomycetes. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591999000100011 |
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